刘四磊，赵允，戴绚丽，吴平

（杭州娃哈哈集团有限公司质量监控部，浙江杭州310018）

摘要：根据JJF1059.1-2012《测量不确定度评定与表示》的原理与方法，建立了高效液相色法测定饮料中酌-氨基丁酸（GABA）含量的测量不确定度评定的数学模型。对数学模型中各不确定度分量的来源进行分析、量化和合成。当取样量为2.0g时，其测定的酌- 氨基丁酸的含量为13.74依0.38mg/L。最终结果的不确定度主要由最小二乘法拟合标准曲线、标准溶液配制、样品处理过程中回收率及样品重复测定引起的。整体评定方法清晰合理，能为实验室在该物质检测过程中进一步提高检测数据的可靠性和一致性提供参考。

关键词：γ- 氨基丁酸；不确定度；高效液相色谱法；饮料

Uncertainty Evaluation for the Determination of γ-Aminobutyric Acid in Beverages by HPLC

LIU Si-lei，ZHAO Yun，DAI Xuan-li，WU Ping

（Hangzhou Wahaha Group Company Limited，Hangzhou 310018，China）

Abstract: Based on JJF1059.1-2012 Evaluation and Expression of Uncertainty in Measurement，a mathematical model wasestablished to assess the uncertainty of analyzing 酌-aminobutyric acid （GABA）in beverages by high performance liquidchromatography（HPLC）. Factors contributing to the uncertainty were analyzed，quantified and synthesized. The expandeduncertainty of GABA determination for 2.0g of samples was 0.38mg/L．The results showed that the uncertainty ofmeasurement was mainly caused by the standard curve fitting by least square method，preparation of standard solution，recovery of sample during processing and the repeat detection of the sample. The evaluation method is clear andreasonable，which can provide a reference for the laboratory to further improve the reliability and consistency of the testdata in GABA testing process.

Key words: γ-aminobutyric acid；uncertainty；high performance liquid chromatography（HPLC）；beverages

（2017年第20卷第4期）