——以番茄汁中克百威的检测为例

卢薇，鲁江*

（湖北工业大学生命科学与健康工程学院，湖北 武汉 430000）

摘要：以番茄汁中克百威的检测为例，将对比教学法应用于高效液相色谱仪（HPLC）和液相色谱-质谱联用仪（HPLC-MS）的实验教学中。在理论教学部分，对比两种大型仪器的原理、构造、应用特点及其在克百威检测中的异同。在上机实操部分，由于番茄汁中的克百威含量低于HPLC 法的检出限但高于HPLC-MS 法的检出限，两种仪器得出截然不同的检测结果。对比教学法切实提高了实验教学效率，提升了学生的大型仪器综合运用能力，对高校应用型人才的高质量培养具有重要意义。

关键词：对比教学法；大型仪器；实验教学；克百威

Application of Comparative Teaching Method in Experimental Teaching of Large-scale Instruments-Takethe Determination of Carbofuran in Tomato Juice as an Example

LU Wei，LU Jiang*

（School of Life and Health Engineering，Hubei University of Technology，Wuhan 430000，China）

Abstract: The comparative teaching method was applied in the experimental teaching of High Performance LiquidChromotography（HPLC）and High Performance Liquid Chromotography-Mass Spectrometry（HPLC-MS）by takingthe determination of carbofuran in tomato juice as an example. In the theoretical teaching part，the similarities anddifferences of the discipline，structure，application in the determination of carbofuran between HPLC and HPLC-MSand their determination of carbofuran were compared. In the practical operation part，different results were given byHPLC and HPLC-MS since the carbofuran content in tomato juice was lower than the detection limit of detection ofHPLC method but higher than that of HPLC-MS method. The teaching efficiency and the comprehensive applicationability of students for large-scale instruments were increased substantially by the comparative teaching method，whichis meaningful to the application-oriented talent cultivation in colleges and universities.

Key words: comparative teaching method；large-scale instrument；experimental teaching；carbofuran

中图分类号：TS20       文献标志码：A        文章编号：1007-7871（2024）01-0051-06

2024，Vol.27，No.1