余家胜1，李 瑞1，杨佳雯1，郭 伟1，吴伟都1,2，吴 琴1,2*
（1 杭州娃哈哈集团有限公司，浙江 杭州 310018；
2 浙江省食品生物工程重点实验室，浙江 杭州 310018）
摘要：本文对无糖可乐中的甜蜜素含量测定方法进行研究，优化了色谱分离条件，建立了离子交换-电导法测定无糖可乐中的甜蜜素的方法。该方法使用的色谱柱是Thermo IonPac AG11-HC（50mm×4mm）保护柱以及ThermoIonPac AS11-HC（250mm×4mm）分离柱，KOH 梯度淋洗，抑制电导检测。结果表明，甜蜜素在0.112mg/L～112mg/L时有良好的线性关系，相关系数在0.999 以上；方法检出限为0.05mg/L，系统相对偏差在2%以内，回收率98.22%～103.9%。该方法操作简便，分析快速，重现性好，结果准确、可靠，适用于无糖可乐中甜蜜素的含量测定。
关键词：离子色谱；电导；无糖可乐；甜蜜素
Determination of Sodium Cyclamate in Diet Coke by Ion Chromatography
YU Jia-sheng1，LI Rui1，YANG Jia-wen1，GUO Wei1，WU Wei-du1,2，WU Qin1,2*
（1 Hangzhou Wahaha Group Co，Ltd，Hangzhou 310018，China；
2 Key Laboratory of Food and Biological Engineering of Zhejiang Province，Hangzhou 310018，China）
Abstract: The study reported the establishment of an ion chromatography method to determine sodium cyclamate indiet coke by ion chromatography using conductivity detector. The separation was achieved on a Thermo IonPac AG11-HC guard column and a Thermo IonPac AS11-HC analytical column with KOH as the gradient eluent and suppressedconductivity detector. Results showed that sodium cyclamate had good liner relations when the concentration was inthe range of 0.112mg/L～112mg/L and the correlation coefficient was above 0.999. The detection limit was 0.05mg/L.The system relative deviation was less than 2%，and the recovery for diet coke was in the range of 98.22%～103.9%.The method is simple，rapid，sensitive，accurate and reproducible. It was suitable for determination of sodiumcyclamate in diet coke.
Key words: ion chromatography；conductivity；diet coke；sodium cyclamate
中图分类号：TS27 文献标志码：A 文章编号：1007-7871（2024）01-0022-05
2024，Vol.27，No.1