郝刚2，杨润1，辛乐仪1，陈润1
（1 西南民族大学生命科学与技术学院，四川成都610041；
2 阿坝州工业经济研究所，四川汶川623000）

摘要：本研究以血红素的纯度为指标，优化了胰蛋白酶水解猪血球的酶解条件，分离提取了猪血中的血红素。猪血球水解的最佳条件是：血红蛋白溶液与酶质量之比80∶3，pH7.5，50℃水解4h，经酸沉离心分离出血红素，纯度为38.74%。将血红素与沙棘汁调配，利用沙棘汁独特的酸甜口感掩盖和弥补血红素的腥味，研制沙棘铁功能强化饮料。经感官实验，确定了沙棘汁铁功能强化饮料最优配方：沙棘汁中血红素浓度0.4g/L，蔗糖120g/L，柠檬酸1g/L，黄原胶0.3g/L，三聚磷酸钠0.5g/L。

关键词：血红素；胰蛋白酶；沙棘；饮料

Extraction of Pig Blood Heme and Development of Sea Buckthorn Iron Functional Fortified Drinks

HAO Gang2，YANG Run1，XIN Le-yi1，CHEN Run1

（1 Southwest Minzu University，College of Life Science and Technology，Chengdu 610041，China；
2 The Research Institute of Industrial Economy of Aba，Wenchuang 623000，China)

Abstract: In this study，the parameter of enzymolysis by trypsin was optimized with the purity of heme as index，and the heme of hog hemoglobin was extracted. The optimum condition of hemoglobin was followed as the mass ratio of hemoglobin solution and enzyme 80∶3，pH7.5，hydrolysis time 4h，50℃. The purity of heme was 38.74% separated by acid precipitation and centrifugation. The sea buckthorn iron functional fortified beverages was advanced with the heme added in sea buckthorn juice，that the fishy smell of heme would be blanketed by the unique sweet and sour taste of sea buckthorn. With the sense experiment，the optimum formulation of sea buckthorn iron functional fortified beverages was confirmed as the concentration of heme 0.4g/L，sucrose 120g/L，citric acid 1g/L，xanthan gum 0.3g/L，sodium tripolyphosphate 0.5g/L to hawthorn juice.

Key words: heme；trypsin；sea buckthorn；beverages

（2019年 第22卷 第6期）